OtherMolecular autism2017

Replicable in vivo physiological and behavioral phenotypes of the Shank3B null mutant mouse model of autism.

Sameer C Dhamne, Jill L Silverman, Chloe E Super and 9 others

PMID 28638591

WHAT IT FOUND

Shank3B knockout mice showed fewer induced seizures, stronger EEG gamma activity, more self-grooming, and some reduced male-female social interaction.

These replicated in two mouse cohorts. This is a preclinical autism model, not a treatment result.

Key findings

01Shank3B knockout mice had fewer PTZ-induced myoclonic seizures than wildtype controls in both cohorts.

02Baseline EEG gamma power was higher in Shank3B knockout mice than wildtype controls in both cohorts.

03Repetitive self-grooming was higher in Shank3B knockout mice than wildtype littermates in both cohorts.

STILL TO COME

How it was doneWhat they found

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What it does not show

The study describes phenotypes in a mouse model and does not report a treatment trial, so it cannot tell a therapist what to do with a patient. The mice were homozygous Shank3B knockouts, whereas the paper notes that the human SHANK3 mutation is generally heterozygous. EEG analyses included only male mice. Several behavioural findings were not consistent across the two cohorts, including anxiety-like behaviour, male sociability on the three-chambered task, and some cognitive tests. Some assays were not repeated in the second cohort because the first cohort showed normal results.

Declared interests

The paper names Autism Speaks and the National Institutes of Health as funders. It also states that the behavioural testing platform was developed with the Autism Speaks Preclinical Autism Consortium for Therapeutics.

The easy way to misread this

Do not read this as evidence that any therapy helps autism symptoms. The paper describes mouse physiology and behaviour, and several behavioural findings were not replicated across cohorts.

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The study

Participants
Behavioural cohorts: 12 male wildtype, 12 female wildtype, 12 male Shank3B knockouts, 10 female Shank3B knockouts in cohort 1; 12 male wildtype, 10 female wildtype, 9 male Shank3B knockouts, 12 female Shank3B knockouts in cohort 2. EEG cohorts: seven male wildtype and eight male Shank3B knockouts in each cohort.
Certainty of evidence
Low

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    Cite

    Sameer C Dhamne, Jill L Silverman, Chloe E Super, et al. Replicable in vivo physiological and behavioral phenotypes of the Shank3B null mutant mouse model of autism. Molecular autism. 2017.

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