OtherAutism research : official journal of the International Society for Autism Research2025

Age-Dependent Effects of Loss of Contactin-Associated Protein-Like 2, an Autism-Associated Gene, on the Acquisition and Recall of Fear Memory.

R J Taugher-Hebl, A Berns, M Jones and 5 others

PMID 40247148

WHAT IT FOUND

In mice modeling autism, loss of the Cntnap2 gene altered fear and anxiety behaviors differently depending on when training occurred.

Knockout mice showed impaired fear memory acquisition post-weaning and hyperactivity at all ages, but anxiety effects varied by developmental stage.

Key findings

01Knockout mice trained after weaning (P30 and P61) showed significantly impaired acquisition of fear memory compared to wild-type mice.

02Knockout mice traveled significantly further than wild-type mice in the elevated zero maze at all ages tested (P17, P29, and P60), indicating hyperactivity.

03Anxiety-related behavior, measured by time spent in closed arms, was significantly lower in juvenile knockout mice (P29) but not in pre-weaning or young adult mice.

STILL TO COME

How it was doneWhat they found

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What it does not show

The study was conducted entirely in mice, so direct application to human clinical practice is not established. The number of mice tested in specific subgroups varied, and some data was excluded due to technical issues or room changes, potentially affecting the robustness of certain anxiety findings. The mechanisms explaining why fear memory deficits appeared at different ages are hypothesized in the discussion but not directly tested in this experiment.

Declared interests

The authors declare no conflicts of interest.

The easy way to misread this

Do not interpret these findings as evidence of how fear or anxiety presents in human patients with ASD. This is a basic science study in mice examining the genetic impact of Cntnap2 loss on neural development, not a clinical trial of a therapy.

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The study

Participants
203 mice
Certainty of evidence
Low

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    Cite

    R J Taugher-Hebl, A Berns, M Jones, et al. Age-Dependent Effects of Loss of Contactin-Associated Protein-Like 2, an Autism-Associated Gene, on the Acquisition and Recall of Fear Memory. Autism research : official journal of the International Society for Autism Research. 2025.

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